The five exons and flanking intronic sequences of PPIB gDNA from the proband were sequenced, and the PPIB mutation was confirmed by restriction-enzyme digestion. This mutation had not been within DNA samples from 115 healthy West African topics or in multiple single-nucleotide-polymorphism databases. In Vitro Biochemical Tests The facts of collagen biochemical assessments in dermal fibroblasts are provided in the Supplementary Appendix. Western blots of cell lysates were probed with antibodies to CyPB , CRTAP, or P3H1 and had been scanned on a LI-COR Odyssey machine.TRANSCRIPTS – The enclosed transcripts are similar to standard university transcripts. Although a copy of the sample transcript will stay in our files, you are suggested by us retain a copy for your records. Suggestion LETTERS – Please maintain these samples for your information, as the University will not keep sample recommendation letters on file. GRADUATE SERVICES – Any noticeable changes or corrections to your articles must be carried out through the Graduate Companies Department only. Always use your student ID number when corresponding around. Please send requests via Email: or Fax: +7-801-992-1502 +44-207-900-1709 VERIFICATIONS – Before responding to any inquiries about your credentials, we attempt to notify you for approval. Therefore, it is in your favor to always keep us informed of your present phone number, fax number and email addresses.